BIOCHEMICAL CHARACTERIZATION OF THE NATIVE BEES OF TWO DIFFERENT ECOSYSTEMS FROM THE CENTRAL FOREST OF PERU

Abstract

With the purpose of characterizing in biochemical form the native bees of the ecosystems of San Ramon and Satipo of the Central Forest of the Peru. 1000 m² has been chosen in each one of them to identify the present nests and to collect samples of quick bees to be born and of the same age in bottles of 50 mililiter containing alcohol to 99 %. The work initiated in April of 2004 and concluded in March of 2005. The biochemical analysis development with technologic of Kloth (1992) for proteins in DiscPAGE system.  Has been a total of 21 nests of native bees, seven in San Ramon  (three Meliponas, three Trigonas, one Plebeia) and 14 in Satipo (Seven Meliponas, five Trigonas, two Plebeias).  Itself selected at random for the electroforetic bullfights, three samples of the ecosystem of San Ramon  (one of Melipona, one of Trigona and one of Plebeia) and six samples of the ecosystem of Satipo (two of Melipona, two of Trigona and two of Plebeia).  we concluded that the individuals of the generous the native bees of both ecosystems, they would be able to correspond to a same species, by the similar characteristics that present the bands of proteins after the electrophoresis. The results show that methodology selected for protein characterization of native bees, allows the separation of bands in genotypes of Melipona, which constitutes a tool that can be used in later works of genetic characterization.