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Cytotoxicity assessment of benzyl isotiocyanate from Troapeolum majus L. breast tumor cell lines
DOI:
https://doi.org/10.26490/uncp.prospectivauniversitaria.2019.16.1021Keywords:
Cytotoxicity, MCF-7, Breast cancer, Benzyl IsiocyanateAbstract
The mastuerzo (Tropaeolum majus L.) is a plant native to Peru, Mexico and parts of Central America. In Peru, It was used since ancient times by our ancestors to combat different diseases. Its components include glucotropaeoline, which when hydrolyzed forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis. Due to this, the aim of the work is to evaluate the cytotoxic effect of benzyl isothiocyanate of mastuerzo exposed to microgravity on the in vitro crop of the MCF7 breast cancer cell line. Extracts of bencil isiothiocyanate from the naturally cultivaded plant were used (T1) and the second were exposed to microgravity at the germination stage (T2), comparing with the commercial product (BI), the cell viability was carried out through the MTT technique making dilutions of 0, 20, 40, 60, 80 and 100 uM of the extract exposed to tumor cell culture of type MCF-7 breast cancer for a time of 12, 24, 48, and 60 hours reading in an ELISA plate reader at 570 nm. Obtaining an LD50 between 22,56 ± 3,24 and 19,97 ± 2,52 at 60 hours, values similar to 48 hours compared to the Benzil control of isiocyanate (BI). In conclusion, no difference were observed between in the effect of the average lethal dose of all treatments in the 48 and 60 hours of incubation an benzyl isiocyanate (BI). The maximum effect of inhibition in all concentrations is appreciable at 60 hours of incubation, stating that there is no significant difference in the biological activities between T1 and T2.
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