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Acid pretreatment of Celery waste (Apium Graveolens)
DOI:
https://doi.org/10.26490/uncp.prospectivauniversitaria.2020.17.1396Keywords:
Celery residues, Acid pretreatment, CelluloseAbstract
The research determined the influence of time, temperature and H2SO4 dose on the characteristics of cellulose and glucose concentration in the acid pretreatment of celery residues, in such a way that the pretreated celery residues have the best conditions for the hydrolysis and subsequent fermentation, and having ethanol as the final product. The experimental part consisted of collecting and preparing the celery residues, preparing the DNS reagent and elaborating the glucose calibration curve that is used to determine the concentration of reducing sugars, carrying out the acid pretreatment to 25 g of residues in each experiment and determining cellulose morphology with the Mira 3 Tescan scanning electron microscope (SEM). The variables that were evaluated are the concentration of H2SO4 (0.1%, 2.5% and 4.0%), temperature (60, 70 and 80 °C) and time (2, 3 and 4 hours) and the experiments of the experimental design were followed. From the initial tests, the low levels of the variables are the best conditions of the acid pretreatment due to the morphology they present that agree with what is indicated in the literature, with this the production of glucose in hydrolysis will be higher and ethanol in fermentation
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